Quantification of Bioactive Compounds in Mentat Tablet (MT)

Quantification of Bioactive Compounds in Mentat Tablet (MT) An ultra-performance liquid chromatography electrospray ionization tandem mass spectrometry method has been developed and validated for simultaneous quantification of twenty-eight major bioactive compounds in Mentat tablet (MT), a complex Indian herbal medicine used in the treatment of neurological disorder and improvement of mental health. Multiple-reaction monitoring scanning was employed for quantification in positive and negative mode. The analysis was accomplished on Waters AQUITY UPLC BEH C18 column with linear gradient elution of water/formic acid (0.1%) and acetonitrile/formic acid (0.1%) at a flow rate of 0.3 mL/min. The proposed method was validated with acceptable linearity (r2, 0.9984-0.9999), precision (RSD, 0.22–2.11%), stability (RSD, 0.16–1.78%) and recovery (RSD ≠¤ 3.74 %), under optimum conditions. The limits of quantitation were low ranging from 0.28 to 3.88 ng/mL for the 28 compounds. The method was successfully applied to the simultaneous deter mination of 28 compounds in twenty batches of MT tablets. Also, hierarchical cluster analysis and principle component analysis was applied to categorize 20 samples based on characteristics of the 28 bioactive compounds. The results have indicated that this advanced method is rapid, sensitive and suitable to show the real quality of the MT composition and also capable of controlling quality of polyherbal formulations having similar markers/raw herbs. Keywords: Multiple Reaction Monitoring / Multi-Marker Quantification / UPLC-ESI-MS/MS / Hierarchical Cluster Analysis / Principle Component Analysis 1. Introduction Herbal medicines (HMs) refer to one herb or complex mixtures, which usually contains hundreds of chemically different components. Their curative effects are principally based on the synergic effect of their multi-targeting and multi-ingredient preparations [1, 2]. Consequently, quality control becomes troublesome for crude drugs. the method that employs pharmacologically active components to evaluate the quality and authenticity of the complex preparations is confronted with severe challenges. Therefore, better analytical strategies to assure their efficacy, safety, and consistency is essential and in great demand [3]. Moreover, the chemical compounds in the poly herbs in HMs products may vary depending on harvest seasons, plant origins, drying processes and other factors. Thus, it seems to be necessary to determine most of the phytochemical constituents of herbal products in order to ensure the reliability and repeatability of pharmacological and clinical research and to enhance pro duct quality control [4, 5]. Currently, selection of a single or a few specific components from a certain herbal medicine as markers for quality assessment is a widely applied strategy. However, it cannot afford sufficient quantitative information for the other medicinal compositions and cannot accurately reflect the quality of HMs products. All the HMs compositions play important roles in the therapeutic effects. Therefore, selecting multiple constituents from different medicinal herbs as evaluation markers has been gradually applied for the quality control of HMs [6, 7]. Mentat tablets (MT, commercial product) is a polyherbal medication with each tablet composed of multiple herbs extracts (listed in Table S1). Major contributed herbs are Bacopa monnieri, Centella asiaticaand Withania somnifera. MT is a unique all-natural multi-ingredient formula that promotes brain health. It improves the mental quotient, memory span, concentration ability, stress threshold and exhibit significant anti-parkinsonian activity. MT also offers protection against convulsions, which is beneficial in insomnia with its sedative and tranquilizing effects [8-10]. Chemically bacosides, saponin mixture in B. monnieri, triterpenoid glycosides in C. asiatica, steroidal lactones in W. somnifera are the major representative ingredients in MT, in comparison to others. [11-14]. Phytochemical investigations show important classes of bioactive constituents in selected plants which are as in combination of MT that are responsible for the bioactivity [15-20]. Literature survey reveals various analytical methods including thin layer chromatography (TLC) [21], high performance thin layer chromatography (HPTLC) [22, 23], liquid chromatography (LC) [6, 24, 25], liquid chromatography coupled with mass spectrometry (LC-MS) [26-29], nuclear magnetic resonance (NMR) [30] for the quantitative analysis of the bioactive constituents in HMs to assess the quality of the complex preparations. To the best of our knowledge, there is no method reported for the simultaneous estimation of selected 28 multi-markers in herbals by UPLC-ESI-MS/MSand no such approach has been explored on this important drug combination for quality and consistency evaluation of this herbal preparation. Natural alteration in preparation processes and climate affects the safety and batch-to-batch uniformity of HMs products. Highly sensitive analytical methods are thus required to identify ingredients and evaluate batch-to-batch variation. Compared to conventional TLC, HPTLC, HPLC method which are less sensitive and takes longer analysis time, UPLC-ESI-MS/MS method in multiple reaction monitoring (MRM) mode is more powerful approach. Due to its rapid separation power, low detection limit, high sensitivity, selectivity and specificity, UPLC-ESI-MS/MS offers effective detection to quantify multi-ingredients in complex sample matrices. Previous methods reported in literature only contained one or few compounds, without the consideration of other active ingredients. This paper describes for the first time a simple, accurate and reliable UPLC-ESI-MS/MS method for the simultaneous determination of 28 multiple bioactive compounds from different polyherbs viz., bacoside A (mixture of bacoside A3, bacopaside II, bacopaside X and bacopasaponin C), withanolide-A, withaferin-A, asiaticoside, madecassoside, jatrorrhizine, palmatine, magnoflorine, curcumin, gallic acid, protocatechuic acid, ferulic acid, caffeic acid, ellagic acid, rosamarinic acid, ursolic acid, catechin, apigenin, luteolin, quercetin, rutin, kaempferol-3-O-rutinoside, corilagin, chrysin and chlorogenic acid with single runtime of 10 min. This method is intended not only for quality control of commercial polyherbal formulated products but also for efficient evaluation of raw materials. The quantitative results were further analyzed by multivariate statistical analysis i.e., hierarchical cluster analysis (HCA) and principle component analysis (PCA) to provide more information about the ch emical differences and batch-to-batch variations. Chemical Structures of all analytes and internal standards were showed in Fig 1. 2. Experimental 2.1 Reagents and materials The reference standards (purity≠¥90%) bacoside A (mixture of bacoside A3, bacopaside II, bacopaside X and bacopasaponin C) was purchased from Natural Remedies Pvt. Ltd. Apigenin, kaempferol-3-O-rutinoside, protocatechuic acid, rosamarinic acid, caffeic acid, ferulic acid, ursolic acid, palmatine, withanolide-A, withaferin-A, asiaticoside, madecassoside, jatrorrhizine, magnoflorine, catechin, chlorogenic acid, curcumin, rutin, corilagin, chrysin, gallic acid and ellagic acid were purchased from Sigma Aldrich Ltd. (St. Louis, MO, USA). Reference standards of quercetin, luteolin and internal standards (IS) andrographolide and piperine were purchased from Extrasyntheses (Genay, France). Twenty different batches of Mentat tablets produced by Himalaya Drug Company, Bangalore, India were purchased from local drug stores from different places in India (Table S2). For all solutions and dilutions, methanol, acetonitrile (LC-MS grade) and formic acid (analytical grade) were purchased from Fluka, Sigma–Aldrich (St. Louis, MO, USA). Milli-Q Ultra-pure water was obtained from a Millipore water purification system (Millipore, Milford, MA, USA). 2.2 Preparation of standard solutions and samples A stock solution containing 28 standards i.e., bacoside A (mixture of bacoside A3, bacopaside II, bacopaside X and bacopasaponin C), withanolide-A, withaferin-A, asiaticoside, madecassoside, jatrorrhizine, palmatine, magnoflorine, curcumin, gallic acid, procatechuic acid, ferulic acid, caeffic acid, ellagic acid, rosamaric acid, ursolic acid, catechin, apigenin, luteolin, quercetin, rutin, kaempferol-3-O-rutinoside, corilagin, chrysin and chlorogenic acid were weighed accurately, dissolved in pure methanol.The working standard solutions were prepared by diluting the mixed standard solution with methanol to a series of concentrations within the ranges from 1 to 1000 ng/mL used for plotting the calibration curves. Meanwhile, each standard was also prepared respectively. The coating of each samples were removed completely, and the remains were smashed into powder. Pulverized sample (0.5g) was weighed precisely, and sonicated by ultrasonicator (53 KHz, Bandelin SONOREX, Berlin) using 50 ml 100% methanol at room temperature for 30 min. The extracted solution was centrifuged at 15000 rpm for 10 min, and the supernatant was filtered through a 0.22  µm syringe filter (Millex-GV, PVDF, Merck Millipore, Darmstadt, Germany) to obtained 10,000  µg/ml. The à ¯Ã‚ ¬Ã‚ ltrates were diluted with methanol to final working solutions and analyzed directly by UPLC-ESI-MS/MS. The internal standards andrographolide for negative mode and piperine for positive mode were spiked to each working concentration of mixed standards solution and sample solution at a final concentration of 50 ng/mL (50  µL of internal standards mixture of 1000 ng/mL of each in methanol) were mixed properly. All solutions were stored at -200C until use and sonicated prior to injection. 2.3 Instrumentation and analytical conditions An Acquity ultra-performance liquid chromatography (UPLCTM) system consisting of an auto sampler and a binary pump (Waters, Milford, MA was used for analysis. The compounds were separated on an Acquity BEH C18 (2.1 mmÃâ€" 50 mm, 1.7 µm; Waters, Milford, MA) analytical column at a column temperature of 25à ¢- ¦C. A gradient elution was achieved using two solvents: 0.1% (v/v) formic acid in water (A) and 0.1% (v/v) formic acid in acetonitrile (B) at a flow rate of 0.3 mL/min. The gradient program consisted of an initial of 5% with linear increase from 5% to 60% B over 1.85 to 7.5 min and increased from 60% B to 90% B over 8.5 min, which was maintained for 1.5 min, followed by a return to the initial condition over 2.5 min with a sample injection volume of 5 µL. The UPLC system was interfaced with hybrid linear ion trap triple-quadrupole mass spectrometer (API 4000 QTRAPâ„ ¢ MS/MS system from AB Sciex, Concord, ON, Canada) equipped with electrospray (Turbo V) ion source. The optimized parameters for positive mode were as follows: the ion spray voltage was set to 5500 V; the turbo spray temperature, 550à ¢- ¦C; nebulizer gas (gas 1), 50 psi; heater gas (gas 2), 50 psi; collision gas, medium; the curtain gas (CUR) was kept at 20 psi. Optimized parameter for negative mode were as follows: the ion spray voltage was set to −4200 V, the turbo spray temperature, 550à ¢- ¦C; nebulizer gas (gas 1), 20 psi; heater gas (gas 2), 20 psi; collision gas, medium; the curtain gas (CUR) was kept at 20 psi. Quantitative analysis was performed using multiple-reaction monitoring (MRM) mode and its conditions were optimized for each compound during infusion. For full scan ESI-MS analysis, the spectra covered the range from m/z 100 to 1000. Analyst 1.5.1 software package (AB Sciex) used for instrument control and data acquisition. The results of the precursor ion, product ion, corresponding declustering potential (DP), entrance potential (EP), collision energy (CE), cell exit potential (CXP) were shown in Table S3. 2.4 Multivariate analysis Hierarchical cluster analysis (HCA) is a tool to identify relatively homogeneous groups of cases based on selected characteristics, using an algorithm that starts with each case in a separate cluster until only one is left. In the experiment, HCA of 20 batches of samples were performed, in which a method called average linkage between groups was employed and 28 markers were selected as the measurement. Similarly, Principal component analysis (PCA) was carried out based on the contents of quantified 28 bioactive compounds in 20 batches of samples. All the experiment was done using software STATISTICA 7.0. When the contents of investigated compounds were below the quantitation limit or not detected in the samples, the values of such elements were considered to be zero.

John Steinbecks Of Mice and Men :: Essays Papers

Of Mice and Men - Critical Evaluation ‘Of Mice and Men’ is a book, which deals with the issues of loneliness, dreams and friendship, the author of this book, is John Steinbeck and it was first published in 1937. The story this book tells is of two friends who travel together looking for work during the recession in America in the 1930’s. The men are George and Lennie. George has to look after Lennie because he is a bit dumb and gets into trouble a lot. They both have a dream of owning their own farm one day. All the characters in the novel show aspects of the main themes, the two main characters, George and Lennie, illustrate these themes throughout the book. They travel together for companionship and for someone to talk to. George and Lennie have nothing in common and therefore the only reason they are friends is to stop each other from becoming lonely. Lennie isn’t all that smart and he worships George utterly, but sometimes George gets annoyed with Lennie’s stupidity and shouts at him, for example, in the first chapter when Lennie was annoying him about ketchup he shouts â€Å"God a’ mighty if I was alone I could live so easy†. Deep down George knows this isn’t true because he doesn’t want to be alone. Also, George cares about Lennie and tries to make sure nothing bad happens to him. Another Character that shows these themes is Crooks. He is isolated from the rest of the workers due to racist prejudice. He lives alone in a shed beside the barn and has very little contact with other people. He is probably the loneliest person on the ranch because know one will speak to him apart form Lennie. He is so lonely he is going insane which is shown when he says â€Å" a guy goes nuts if he ain’t got nobody, don’t make no difference who the guy is, long’s he’s with you. I tell ya†. This really sums up Crooks feelings, all he wants is someone to talk to. On a broader scale the whole structure of the book gives the impression of loneliness. For example, the story is set in a town called Soledad, which is Spanish for loneliness. Also the first chapter starts by giving you a detailed description of the surroundings, the landscape of the Salinas River and â€Å"the strong and stony Gabilan mountains†. John Steinbeck's Of Mice and Men :: Essays Papers Of Mice and Men - Critical Evaluation ‘Of Mice and Men’ is a book, which deals with the issues of loneliness, dreams and friendship, the author of this book, is John Steinbeck and it was first published in 1937. The story this book tells is of two friends who travel together looking for work during the recession in America in the 1930’s. The men are George and Lennie. George has to look after Lennie because he is a bit dumb and gets into trouble a lot. They both have a dream of owning their own farm one day. All the characters in the novel show aspects of the main themes, the two main characters, George and Lennie, illustrate these themes throughout the book. They travel together for companionship and for someone to talk to. George and Lennie have nothing in common and therefore the only reason they are friends is to stop each other from becoming lonely. Lennie isn’t all that smart and he worships George utterly, but sometimes George gets annoyed with Lennie’s stupidity and shouts at him, for example, in the first chapter when Lennie was annoying him about ketchup he shouts â€Å"God a’ mighty if I was alone I could live so easy†. Deep down George knows this isn’t true because he doesn’t want to be alone. Also, George cares about Lennie and tries to make sure nothing bad happens to him. Another Character that shows these themes is Crooks. He is isolated from the rest of the workers due to racist prejudice. He lives alone in a shed beside the barn and has very little contact with other people. He is probably the loneliest person on the ranch because know one will speak to him apart form Lennie. He is so lonely he is going insane which is shown when he says â€Å" a guy goes nuts if he ain’t got nobody, don’t make no difference who the guy is, long’s he’s with you. I tell ya†. This really sums up Crooks feelings, all he wants is someone to talk to. On a broader scale the whole structure of the book gives the impression of loneliness. For example, the story is set in a town called Soledad, which is Spanish for loneliness. Also the first chapter starts by giving you a detailed description of the surroundings, the landscape of the Salinas River and â€Å"the strong and stony Gabilan mountains†.

Prison Reform Essay

Reform was a major issue in early 19th century America because it was a time when more middle-classed Americans were able to devote time to social causes and issues that they saw that concerned them. One of these important social movements was prison reform, and how men and women in prison were treated. In the early 1800’s the United States was regarded as having the best penal system in the world. This is why during the early 1800’s Alexis de Tocqueville was sent from France to the United States to study the penal system there. What he was coming here to study was how the system of prisons had been reformed already by Americans. Many individuals, in particular religious advocates who took up the cause of prison reform, had established themselves as the voice of prisoners. They felt that while prisoners needed to do time for their crimes and be punished accordingly, they also had the right to have good conditions within the prisons themselves. These people had a goal of creating prisons that were conducive to not only punishing individuals, but reforming them became an important part of their time in prison. These religious reformers felt that prisoners should be reformed to become good citizens and, if they never left jail, then at least they could be religious individuals.   Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   The successes of these reformers were that they did create a great amount of interest in reform. Many new prisons began to be built that would be more conducive to reforming prisoners. These successes were shortly lived, however, because sadly the prisons did not go up as quickly as the prisoners were going in and therefore old prisons that were not good conditions were still in use, and many prisoners still languished in them, in even worse conditions than before.

Jacks Multiple Personalities in Fight Club

Jacks Multiple Personalities in Fight Club Free Online Research Papers When we first meet the main character, only identified as Jack, he has a gun to his head battling his split personality, Tyler Durdan. He then takes you back many months so you can know how it is he came to that state. Jack is a 30 year old single white male complaining of insomnia for over 6 months. His job as a liability consultant for an automotive company requires him to take frequent trips to different time zones, usually on a short notice, therefore leaving him jet lagged. This issue has endured for at least six months. He states he is in pain and wants something to just make him sleep. Jack states that on several occasions he has woken up in strange places and does not know how or when he got there. He realized that having blackouts (not due to substance abuse or general medical condition) was disturbing and unusual behavior. The doctor he visited dismisses his claims and suggests he attend a support group for cancer to witness real pain. An image of his alter-ego briefly appears at that moment. Due to his job, Jack does not have any immediate friends or family. He refers to the passengers on the planes as his single serving friend. Jack begins attending a variety of support groups for ailments that he does not have in order to do something during his bouts of insomnia. After the first few meetings, Jacks insomnia seemed to elapse and he uses the meetings as his sleeping pill. His recovery is short lived due to another fake survivor, Marla Singer. Her presence in his groups threatened his sleep because she knows that he is faking. The patient is coherent and can fully comprehend what is going on around him. His dress is appropriate however his clothes are unkempt. His hair is disheveled and dirty. He appears to look anxious with dark circles under his eyes and not shaven in a couple of days. His posture is slumped. PRESENTING PROBLEM: While on a plane returning from a business trip Jack meets Tyler Durdan, his split personality. Tyler is the complete opposite of Jack. He is everything that Jack wishes he could be. With his red tinted sunglasses and leather jacket he resembles more of a rock star as compared to Jacks drab wardrobe of white button up shirts and brown ties. Jack seems intrigued about Tyler and claims he is the most interesting single serving friend that he has ever met. When Jack returns to his condo he finds out that his apartment has exploded. All of his prize possessions, his IKEA furniture, have been destroyed. He feels pathetic of the fact that when they recover his belongings they will only find condiments in his refrigerator, thus exposing that he had no life. Jack called onto his inner self, Tyler to help rescue him from his dismal life. Jack retreats to a vacant house in the middle of no where and that is where the relationship between Jack and Tyler formulate. Initially, Jack is unaware tha t Tyler is not real. This is shown by the fact that he refers to himself as us, engages in conversions, fist fights with Tyler and at one point even sharing a beer with Tyler. SITUATIONS STRESSORS: Jack feels unsatisfied with his life. He feels abandoned by friends and most importantly his family. His father deserted the family when he was a little boy. His career leaves him discontented and unfulfilling. After the explosion of his condo his old personality, Jack, began to digress and Tyler Durdan was brought to surface. The initial meeting with Tyler ends in an altercation attack. Tyler encourages Jack to hit him, which in reality is really him. He has a physical fist fight with himself in the parking lot, leaving him badly bruised externally however emotional healed. The fighting allowed him to release all of his pent up frustrations with his life. From this emerged Fight Club. Fight Club was a place where men could go and fight each other to let it all go. Theyre fighting was a way to relieve stress for their cruel lives. These men felt refreshed and saw it as therapy. MENTAL STATUS EXAM: During the duration of the movie Jacks appearance continued to deteriorate. His clothes became more tousled. He went to work looking as if he didnt take a bath, wash his hair or shave, the circles under his eyes got darker and heavier. His shirts were often stained with blood and his face was always bruised from the fights. His boss noted his appearance and suggested he take time off to get himself together. Although Jack physically looked horrible, his work performance did not suffer. He would turn in his assignments on time and complete. When Tyler was in charge of the body he engaged in a sexual relationship with one time nemesis, Marla Singer. When Jacks personality would reappear after an encounter with her he would be verbally hostile towards her and kick her out of his house. Marla, who was a self destructive person to begin with, continued this relationship despite the verbal abuse she received from Jack. Jack continued to participate in Fight Club for over a year. He no longer needed the support groups to feel good about himself. The clubs grew from a few members, to hundreds, even thousands. As the Fight Clubs expanded, so did the destruction. It went from men fighting in the basement of a bar to vandalizing buildings, cars and other symbols of repression by society. In a sense, Fight Club turned into a mini-army of men who felt repressed by the government. Most of the men were blue collar workers who seemed as if they could never get ahead in life. They idealized Tyler Durdan and looked up to him as if he were God. They terrorized cities in his honor. The exposure from the media only fueled their zeal for destruction. As Fight Club grew so did the persona of Tyler Durdan. When Jack noticed these men were giving credit for their new found happiness only to Tyler, he felt jealous and rejected. SYMPTOMS OF IMPAIRED FUNCTIONING: Jacks bouts with insomnia, violent behavior, and black outs, interrupted his normal day to day living. When Jack had his insomnia, Tylers personality took over and got jobs where he could interject subtle doses of shock to people. For instance, he worked as a waiter where he urinated in the food. He also worked at a movie theater changing the film. Instead of just changing the rolls of film, he cut and pasted snippets of pornographic images into the film. The people viewing the movies only saw a snapshot of a naked person for less than a second. Jacks aggression carried over into his work place. When his boss became annoyed with his unkempt appearance, absenteeism and apathetic attitude he fired him. Instead of responding rational, Jack beat himself up in his bosss office and blackmailed by threatening to expose company corruption or to keep him on as an outside consultant in exchange for his silence. This afforded him and Tyler with more time to devote to Fight Club to participate i n the expansion in different cities and states. Most times when Jack involved himself with Tyler Durdan he was aware of what actions that Tyler was doing. He usually was a silent bystander while Tyler masterminded mayhem. Other times he would have outright conversations with Tyler in front of others, questioning his actions. The only times Jack was not conscious of what was going on around him was when he was with Marla Singer or traveling to recruit new members. PATIENTS STRENGTHS AND ASSETS: Even though Tyler Durdan had no respect for others, experienced no fear or regrets, Jacks caring disposition makes him rational in chaotic situations. He understands the difference between whats right and wrong. When Fight Club escalated to the mayhem that erupted on the city, Jack did not feel comfortable with it. He questioned Tylers intent; however, Tyler did not want Jack to be involved. He stated that Jack determined his own level of involvement with him. His regard for other people is evident when one the Fight Club members, Bob, was killed during a botched vandalism attempt. The other members of the club wanted to bury him in the back yard as if he were just evidence of their devious acts. Jack had to remind the men that he was a human being with feelings not just a disregarded object. Another example of Jacks softer side was obvious when he felt that Marla was in danger. He attempted to rescue her by putting her on a bus away from harms way. Tyler did not want Jack to discuss him or his actions to anyone, especially her. When the lines between Tyler and Jack started to get blurry, he went to Marla to ask her questions about Tyler. Once this door was opened, Tyler Durdan wanted to get rid of Marla to prevent Jack from seeking out more answers about reality. Tyler felt as though, once Jack knew that Tyler was really within him and causing all this havoc that he would retreat back to his old boring self. It is clearly apparent that Jack possess excellent organizational skills and leadership ability. The way he evolved fight club from a bunch of guys just fighting into an a group of soldiers conducting calculated terrorist attacks to the city was more on the lines of a General in the army instead of a Liability Consultant. Even though Tyler Durdans persona was the mastermind behind the coordination, the ability to do so came from deep within Jack. DIAGNOSIS: AXIS I: NONE AXIS IV: NONE AXIS II: DISSOCIATIVE IDENTITY DISORDER AXIS V: GAF SCORE-30 AXIS III: NONE DIAGNOSTIC COMMENTS: Jacks life was unfulfilling and unsatisfying. He had no friends or family. He lived alone and the only contact to the outside world was his job. Jack wanted a change in his life. The personality of Tyler Durdan was created to make Jack become the person he wished he could be. He created the other self to free himself from his bleak world. However, Jack was oblivious to the fact that he indeed was creating an alternate personality. After a car accident that Tyler caused, which Tyler Durdan referred to as a near life experience, he had a revelation and that is when it became apparent to him that he and Tyler were undoubtedly one in the same. Tyler Durdans philosophy on life was live life like you were dying while Jack was cautious and calculated. Since he worked as a Liability Consultant for an automotive company he saw the danger in everything. The split between the conscious happened once Jack was completely frustrated at how his life at 30 years old turned out. He intentionally blew up his condo to start fresh. However, when his alter-ego turned out to be more than he was willing to handle that is when he decided to wipe him out. He did this by attempting to kill himself in order to get rid of Tyler Durdan. Fortunately, he only killed Tyler Durdan not himself. TREATMENT GOALS Learn to disentangle Jack from his alternate personality Boost Self Esteem Teach Jack how to deal with situation stressors rationally Teach Jack how to control impulses Learn about healthy defense mechanisms TREATMENT PLAN Refer to a psychiatrist for long term individual therapy Recommend medication for depression or antic-psychotic behavior Hypnotherapy Research Papers on Jack's Multiple Personalities in Fight ClubThe Fifth HorsemanThe Spring and AutumnPersonal Experience with Teen PregnancyWhere Wild and West MeetThe Masque of the Red Death Room meaningsNever Been Kicked Out of a Place This NiceMind TravelArguments for Physician-Assisted Suicide (PAS)Effects of Television Violence on Children19 Century Society: A Deeply Divided Era

The Community Employment Innovation Project (CEIP)

The Community Employment Innovation Project (CEIP) Advertising We will write a custom essay sample on The Community Employment Innovation Project (CEIP) specifically for you for only $16.05 $11/page Learn More Description of the logic model process The CEIP needs to address unemployment in communities around Cape Breton Island in Nova Scotia. The need for the CEIP is to develop the local capacity, initiate community-based employment, develop new skills, enhance job placement, and work-related contacts with a long-term goal of improving employment success and reducing unemployment. The CEIP targets community and individuals for a membership of 1,500 participants. Both community and individuals will take action to participate in identifying viable projects at the community level. Participants are mainly volunteers who receive fiscal support and other supports from Human Resource Development Canada and the Nova Scotia Department of Community Services. These activities will lead to ambitious and long-term outcom es, such as building local capacity, enhancing employment prospects, reducing unemployment and long work hours, and improving quality of life. The short-term and mid-term outcomes will ensure that the community and individuals realize immediate benefits and associated outcomes of the program. All stakeholders must work together throughout various stages in order to realize these benefits (McDavid, Huse and Hawthorn, 2013). The program activities included training alongside assessment of aptitudes, experiences, education, and skills. It also included skill development, funding, and capacity building, surrendering EI and IA for community wage, identifying suitable community-based projects, recruiting 1,500 volunteers, and developing work-related networks.Advertising Looking for essay on public administration? Let's see if we can help you! Get your first paper with 15% OFF Learn More The program outputs included all completed activities. These are tangible deliv erables because of activities. The CEIP activities of engaging 1,500 volunteers and community members, funding, and surrendering EI and IA have corresponding outputs on the number of community-based projects identified, participation, ongoing enrolment, comparison sites, and the number of volunteers who surrender EI and IA for community wages. The program inputs or resources included human resources in terms of individuals and community volunteers, CEIP trainers, and program coordinators. There were fiscal resources for the program from the Human Resource Development Canada and the Nova Scotia Department of Community Services and facilities for developing local capacity, skills, and knowledge. The CEIP is an ongoing program that started in 1999. Today, it is in the evaluation stage. The stage would show the realized program outcomes and any factors, which have facilitated or hindered the realization of these outcomes. The program contextual factors are the beliefs that communities a nd individuals know their challenges and can develop the best methods to solve them. At the same time, the focus on reducing employment and long-term employment success are the mainframe contexts that drive CEIP. Reference McDavid, J. C., Huse, I., and Hawthorn, L. R. L. (2013). Program Evaluation and Performance Measurement: An Introduction to Practice. Thousand Oaks: Sage Publications, Inc.

The place of the doctrine of parliamentary sovereignty in the Assignment

The place of the doctrine of parliamentary sovereignty in the government of the United Kingdom has changed in recent years and - Assignment Example This concept consists of two cardinal principles. First, Parliament has the power to make or annul any law irrespective of its nature. Second, no other agency, entity, or person has the power or right to override or annul the statutes made by Parliament (Warren, 2008, p. 3). In Britain, the sovereignty of Parliament has been reviewed in domestic and international contexts. As a member of the European Union, Britain had to permit the incursion of the laws of the European Council. This required Britain to review its domestic laws, on a regular basis, so as to ensure that they were compatible with the European Council (EC) law and Britain’s international obligations (Ginsburg, 2003, p. 3). As a result of these developments, the national courts in Britain have to interpret domestic law in the light of EC law. An important constituent of the common law is the fundamental tenet of legislative supremacy. Nevertheless, it would be erroneous to describe it as being inviolable. In fact, there are a number of precedents that inexorably establish that legislative supremacy can be altered by judicial decisions and Acts of Parliament. Consequently, it is imprudent to vehemently assert the supremacy of Parliament (Bradley & Ewing, 2007, p. 78). The inexorable incursions of European integration have significantly diluted the Diceyan principle of legislative supremacy. Thus, the courts, consequent to empowerment by the Human Rights Act 1998, are nearly proximate to a position, whereby they can rescind an Act of Parliament. Moreover, the introduction of the principle of devolution implies that legislation is not the sole preserve of Westminster (Bradley & Ewing, 2007, p. 78). In the UK, an Act of Parliament had precedence over any other international law, under the doctrine of Parliamentary sovereignty. This was the situation in the UK prior to its becoming a member to the European Union. The enactment of the European Communities Act 1972 changed this situation by making the national laws subordinate to EC law (Fabunmi & Araromi, 2009, p. 198). As such, the European Communities Act 1972 obliged the Member States of the European Union to circumscribe their sovereign rights. This was the outcome of their having to transfer power from their domestic legal system to the EC. Thus, EC law takes precedence over domestic law (Fabunmi & Araromi, 2009, p. 198). Furthermore, every Member State has to implement the provisions of the Treaties and give legal effect to them, without any deviation. Consequently, the UK Parliament is constrained by the European Communities Act to enact laws that are compatible with Community Law. Specifically, section 2(1) of the European Communities Act 1972, precludes Parliament from making laws that are incompatible with Community Law. Furthermore, the provisions of this section make it obligatory for Parliament to give effect to the EC Treaties in its legislation. Thus, EC Law has to be recognised and implemented. Membership of the European Union caused the UK to change its system of weights and measures to the metric system. This was greeted with considerable hostility among the public and culminated in the Thoburn v Sunderland City Council or metric martyrs’ case. In its ruling the Court of Appeal opined that the Parliamentary sovereignty was seriously impacted by the European Communities Act 1972. This specific Act had engendered the harmonisation of the domestic legislation of

Leadership Experience Essay Example | Topics and Well Written Essays - 1500 words

Leadership Experience - Essay Example The team managed the lowest price and finished the task within the three minutes. This paper seeks to describe the activity, what was learnt, and the solutions provided with a focus on communication, team dynamics, influencing other team members, sharing information, agreeing on tasks and objectives. 1. Leadership, strategy and upward communication Strategic leadership can be defined as the ability to maintain flexibility, anticipate, and empower the other team members to form necessary strategic change. It involves coping with increasing changes, management of an entire team rather than functional subunits, and management of activities through the other team members. As a team leader, I had to affect the behavior of the other team members in environments that were sometimes uncertain via personal example and ability to envision the requirements for the next stage in the task (Yukl, 2010 p34). It is not feasible to formulate strategies which will give returns without strategic leader s. Therefore, it is necessary that as, a team leader, I had the ability to be a strategic leader to avoid over managing and poorly leading the team. Effective, strategic leadership shapes the formation of strategic mission and intent which influence successful strategic actions. These actions result in the formulation and implementation of these strategies which then yield strategic competitiveness and above average returns. For a leader to prepare the team’s minds towards one vision and direction, it is necessary to transform the team’s strategic direction. Directing the team towards the set goals, and having the ability to adapt concurrently ensures that the strategy delivers the aim that the leader wants (Yukl, 2010 p35). It is essential to have a different way of thought on how resources can be marshaled in the formulation and execution of the strategy in order to be successful. This balances strategy making’s human dimension with analytical perspectives tha t are focused. Coupled with time management commitments in engaging the team in dialogue on strategy, this strategy lays the foundation for building a foundation for creation of winning teams that define, adjust, commit, and adapt quickly to strategy. I learnt that in order to shape the strategic direction, I had to know what my primary role was as the chief strategist, my role as a leader during the strategy making process, the team required for the strategy making, and the point at which the making of the strategy can be considered finished. A leader requires to incorporate human and analytical dimensions, in order to create winning outcomes, and for the team to comprehend and commit to the set common agenda, and must also be clear about each member’s strategic capacity. Using feedback from the team members helps to provide a holistic view on the team’s and its individual member’s effectiveness. Ratings by the team members themselves and their peers can be use d to evaluate the efficiency of the team and the individual (Yukl, 2010 p35). The members rate the following personal traits and competencies: Communication which is the ability to objectively, supportively, and openly share opportunities using nonverbal and verbal behaviors while listening actively. Conflict resolution, which involves the